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primary antibodies against toll-like receptor 4 (tlr4)  (Beyotime)


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    Beyotime primary antibodies against toll-like receptor 4 (tlr4)
    Primary Antibodies Against Toll Like Receptor 4 (Tlr4), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against toll-like receptor 4 (tlr4)/product/Beyotime
    Average 90 stars, based on 1 article reviews
    primary antibodies against toll-like receptor 4 (tlr4) - by Bioz Stars, 2026-03
    90/100 stars

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    ( A ) The putative binding site of miR-140-5p and <t>TLR4</t> is shown. ( B ) qRT-PCR analysis was used to assess the expression levels of TLR4 in ten kidney tissues from patients with DN and ten kidney tissues from healthy controls. P <0.01, vs. control group. ( C ) Spearman’s correlation was used to assess the correlation of miR-140-5p and TLR4 expression in kidney tissues from DN patients (r: −0.8183; P <0.01). ( D ) HK-2 cells were transfected with miR-140-5p mimics and miR-140-5p inhibitor for 24 h, and the cells were harvested. Then, the protein levels of TLR4 were analyzed by Western blot. ( E ) HK-2 cells were co-transfected with firefly luciferase constructs containing the TLR4 wild-type or mutated 3′-UTRs and miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC, and then the luciferase activity was analyzed using the dual-luciferase reporter assay system. Data represent the mean ± SD of three independent experiments. ** P <0.01 vs mimics NC; ## P <0.01 vs inhibtor NC. ( F ) HK-2 cells were transfected with miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC for 24 h and then exposed to HG for 24 h, the expression of TLR4 was measured by qRT-PCR. Data represent the mean ± SD of three independent experiments. * P <0.05, ** P <0.01 vs control group; ## P <0.01 vs HG+ mimics NC; && P <0.01 vs HG + inhibitor NC.
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    ( A ) The putative binding site of miR-140-5p and <t>TLR4</t> is shown. ( B ) qRT-PCR analysis was used to assess the expression levels of TLR4 in ten kidney tissues from patients with DN and ten kidney tissues from healthy controls. P <0.01, vs. control group. ( C ) Spearman’s correlation was used to assess the correlation of miR-140-5p and TLR4 expression in kidney tissues from DN patients (r: −0.8183; P <0.01). ( D ) HK-2 cells were transfected with miR-140-5p mimics and miR-140-5p inhibitor for 24 h, and the cells were harvested. Then, the protein levels of TLR4 were analyzed by Western blot. ( E ) HK-2 cells were co-transfected with firefly luciferase constructs containing the TLR4 wild-type or mutated 3′-UTRs and miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC, and then the luciferase activity was analyzed using the dual-luciferase reporter assay system. Data represent the mean ± SD of three independent experiments. ** P <0.01 vs mimics NC; ## P <0.01 vs inhibtor NC. ( F ) HK-2 cells were transfected with miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC for 24 h and then exposed to HG for 24 h, the expression of TLR4 was measured by qRT-PCR. Data represent the mean ± SD of three independent experiments. * P <0.05, ** P <0.01 vs control group; ## P <0.01 vs HG+ mimics NC; && P <0.01 vs HG + inhibitor NC.
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    ( A ) The putative binding site of miR-140-5p and <t>TLR4</t> is shown. ( B ) qRT-PCR analysis was used to assess the expression levels of TLR4 in ten kidney tissues from patients with DN and ten kidney tissues from healthy controls. P <0.01, vs. control group. ( C ) Spearman’s correlation was used to assess the correlation of miR-140-5p and TLR4 expression in kidney tissues from DN patients (r: −0.8183; P <0.01). ( D ) HK-2 cells were transfected with miR-140-5p mimics and miR-140-5p inhibitor for 24 h, and the cells were harvested. Then, the protein levels of TLR4 were analyzed by Western blot. ( E ) HK-2 cells were co-transfected with firefly luciferase constructs containing the TLR4 wild-type or mutated 3′-UTRs and miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC, and then the luciferase activity was analyzed using the dual-luciferase reporter assay system. Data represent the mean ± SD of three independent experiments. ** P <0.01 vs mimics NC; ## P <0.01 vs inhibtor NC. ( F ) HK-2 cells were transfected with miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC for 24 h and then exposed to HG for 24 h, the expression of TLR4 was measured by qRT-PCR. Data represent the mean ± SD of three independent experiments. * P <0.05, ** P <0.01 vs control group; ## P <0.01 vs HG+ mimics NC; && P <0.01 vs HG + inhibitor NC.
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    ( A ) The putative binding site of miR-140-5p and <t>TLR4</t> is shown. ( B ) qRT-PCR analysis was used to assess the expression levels of TLR4 in ten kidney tissues from patients with DN and ten kidney tissues from healthy controls. P <0.01, vs. control group. ( C ) Spearman’s correlation was used to assess the correlation of miR-140-5p and TLR4 expression in kidney tissues from DN patients (r: −0.8183; P <0.01). ( D ) HK-2 cells were transfected with miR-140-5p mimics and miR-140-5p inhibitor for 24 h, and the cells were harvested. Then, the protein levels of TLR4 were analyzed by Western blot. ( E ) HK-2 cells were co-transfected with firefly luciferase constructs containing the TLR4 wild-type or mutated 3′-UTRs and miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC, and then the luciferase activity was analyzed using the dual-luciferase reporter assay system. Data represent the mean ± SD of three independent experiments. ** P <0.01 vs mimics NC; ## P <0.01 vs inhibtor NC. ( F ) HK-2 cells were transfected with miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC for 24 h and then exposed to HG for 24 h, the expression of TLR4 was measured by qRT-PCR. Data represent the mean ± SD of three independent experiments. * P <0.05, ** P <0.01 vs control group; ## P <0.01 vs HG+ mimics NC; && P <0.01 vs HG + inhibitor NC.
    The Primary Antibody Against Toll Like Receptor 4 Tlr4(1:50 Diluted, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the primary antibody against toll-like receptor 4 tlr4(1:50 diluted/product/Santa Cruz Biotechnology
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    ( A ) The putative binding site of miR-140-5p and TLR4 is shown. ( B ) qRT-PCR analysis was used to assess the expression levels of TLR4 in ten kidney tissues from patients with DN and ten kidney tissues from healthy controls. P <0.01, vs. control group. ( C ) Spearman’s correlation was used to assess the correlation of miR-140-5p and TLR4 expression in kidney tissues from DN patients (r: −0.8183; P <0.01). ( D ) HK-2 cells were transfected with miR-140-5p mimics and miR-140-5p inhibitor for 24 h, and the cells were harvested. Then, the protein levels of TLR4 were analyzed by Western blot. ( E ) HK-2 cells were co-transfected with firefly luciferase constructs containing the TLR4 wild-type or mutated 3′-UTRs and miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC, and then the luciferase activity was analyzed using the dual-luciferase reporter assay system. Data represent the mean ± SD of three independent experiments. ** P <0.01 vs mimics NC; ## P <0.01 vs inhibtor NC. ( F ) HK-2 cells were transfected with miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC for 24 h and then exposed to HG for 24 h, the expression of TLR4 was measured by qRT-PCR. Data represent the mean ± SD of three independent experiments. * P <0.05, ** P <0.01 vs control group; ## P <0.01 vs HG+ mimics NC; && P <0.01 vs HG + inhibitor NC.

    Journal: Bioscience Reports

    Article Title: MicroRNA-140-5p ameliorates the high glucose-induced apoptosis and inflammation through suppressing TLR4/NF-κB signaling pathway in human renal tubular epithelial cells

    doi: 10.1042/BSR20192384

    Figure Lengend Snippet: ( A ) The putative binding site of miR-140-5p and TLR4 is shown. ( B ) qRT-PCR analysis was used to assess the expression levels of TLR4 in ten kidney tissues from patients with DN and ten kidney tissues from healthy controls. P <0.01, vs. control group. ( C ) Spearman’s correlation was used to assess the correlation of miR-140-5p and TLR4 expression in kidney tissues from DN patients (r: −0.8183; P <0.01). ( D ) HK-2 cells were transfected with miR-140-5p mimics and miR-140-5p inhibitor for 24 h, and the cells were harvested. Then, the protein levels of TLR4 were analyzed by Western blot. ( E ) HK-2 cells were co-transfected with firefly luciferase constructs containing the TLR4 wild-type or mutated 3′-UTRs and miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC, and then the luciferase activity was analyzed using the dual-luciferase reporter assay system. Data represent the mean ± SD of three independent experiments. ** P <0.01 vs mimics NC; ## P <0.01 vs inhibtor NC. ( F ) HK-2 cells were transfected with miR-140-5p mimics, mimics NC, miR-140-5p inhibitor or inhibitor NC for 24 h and then exposed to HG for 24 h, the expression of TLR4 was measured by qRT-PCR. Data represent the mean ± SD of three independent experiments. * P <0.05, ** P <0.01 vs control group; ## P <0.01 vs HG+ mimics NC; && P <0.01 vs HG + inhibitor NC.

    Article Snippet: Primary antibodies against TLR4 (cat. no. 14358; 1:2000), nuclear p-p65 (cat no. #3033, Cell Signaling Technology, 1:1000 dilution), phosphorylated IκB-α (p-IκB-α; cat no. #2859, Cell Signaling Technology, 1:1000 dilution), IκB-α (cat no.sc-52900, Santa Cruz Biotechnology, 1:1000 dilution), Histone H3 (cat no. #9728, 1:2000, Cell Signaling Technology, 1:1000 dilution) and β-actin (cat no.#4970, Cell Signaling Technology, 1:2000 dilution) were incubated at 4°C overnight.

    Techniques: Binding Assay, Quantitative RT-PCR, Expressing, Control, Transfection, Western Blot, Luciferase, Construct, Activity Assay, Reporter Assay